| 1 | Am. J. Med. Genet. B Neuropsychiatr. Genet. 2005 Feb 133B: 1-5 |
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| PMID | 15645418 |
| Title | Assessing the validity of blood-based gene expression profiles for the classification of schizophrenia and bipolar disorder: a preliminary report. |
| Abstract | Recent advances have facilitated the use of blood-derived RNA to conduct genomic analyses of human diseases. This emerging technology represents a rigorous and convenient alternative to traditional tissue biopsy-derived RNA, as it allows for larger sample sizes, better standardization of technical procedures, and the ability to non-invasively profile human subjects. In the present pilot study, we have collected RNA from blood of patients diagnosed withschizophreniaor bipolar disorder (BPD), as well as normal control subjects. Using microarray analysis, we found that each disease state exhibited a unique expressed genome signature, allowing us to discriminate between theschizophrenia, BPD, and control groups. In addition, we validated changes in several potential biomarker genes forschizophreniaand BPD by RT-PCR, and some of these were found to code to chromosomal loci previously linked toschizophrenia. Linear and non-linear combinations of eight putative biomarker genes (APOBEC3B, ADSS, ATM, CLC, CTBP1, DATF1,CXCL1, and S100A9) were able to discriminate betweenschizophrenia, BPD, and control samples, with an overall accuracy of 95%-97% as indicated by receiver operating characteristic (ROC) curve analysis. We therefore propose that blood cell-derived RNA may have significant value for performing diagnostic functions and identifying disease biomarkers inschizophreniaand BPD. |
| SCZ关键字 | schizophrenia |
| 2 | J Psychiatr Res 2008 Jul 42: 639-43 |
| PMID | 17825842 |
| Title | Verification of proposed peripheral biomarkers in mononuclear cells of individuals with schizophrenia. |
| Abstract | Recent studies reported gene expression alterations in peripheral blood cells (PBC) obtained from patients withschizophreniaas compared to healthy controls. These alterations can not only be regarded as potential biomarkers but can also further our understanding of the disease. In light of previous reports, expression levels of the following genes: APOBEC3B,CXCL1, DRD2, GNAO1, Kir2.3, S100A9, and SELENBP1 in PBCs were compared between 30 first-hospitalized patients withschizophrenia和26个使用定量实时PCR的健康对照。确认了来自基因的转录本的显着海拔（2.6倍； p <0.05）CXCL1but not from the other genes investigated. Within the patients group, APOBEC3B expression was inversely correlated with duration of neuroleptic treatment. These findings indicate that gene expression in PBC from patients withschizophreniamay not only vary with the methods used for analysis but also with state-related differences in gene expression. |
| SCZ关键字 | schizophrenia |
| 3 | Prog. Neuropsychopharmacol. Biol. Psychiatry 2015 Mar 57: 155-65 |
| PMID | 25445065 |
| Title | Behavioral alterations in rat offspring following maternal immune activation and ELR-CXC chemokine receptor antagonism during pregnancy: implications for neurodevelopmental psychiatric disorders. |
| Abstract | Research suggests that maternal immune activation (MIA) during pregnancy increases the risk of neurodevelopmental disorders includingschizophreniaand autism in the offspring. Current theories suggest that inflammatory mediators including cytokines and chemokines may underlie the increased risk of these disorders in humans. For example, elevated maternal interleukin-8 (IL-8) during pregnancy is associated with increased risk ofschizophrenia的后代。鉴于这种协会,the present experiments examined ELR-CXC chemokinesCXCL1和CXCL2，人IL-8的啮齿动物同源物以及其受体的激活（CXCR1和CXCR2）在已建立的MIA啮齿动物模型中。在妊娠天15.使用多重分析的蛋白质分析和ELISA的蛋白质分析表明，Polyi：C显着提高了脑膜内属的母体血清浓度-1？，肿瘤坏死因子和CXCL13h after administration. Subsequent experiments tested the role of elevated maternalCXCL1on behavior of the offspring by administering a CXCR1/CXCR2 antagonist (G31P; 500 ?g/kg, i.p.; 1h before, 48 and 96 h after polyI:C treatment). The male offspring of dams treated with polyI:C demonstrated subtle impairments in prepulse inhibition (PPI), impaired associative and crossmodal recognition memory, and altered behavioral flexibility in an operant test battery. While G31P did not completely reverse the behavioral impairments caused by polyI:C, it enhanced PPI during adolescence and strategy set-shifting and reversal learning during young adulthood. These results suggest that while polyI:C treatment significantly increases maternalCXCL1, elevations of this chemokine are not solely responsible for the effects of polyI:C on the behavior of the offspring. |
| SCZ关键字 | schizophrenia |