| 1 | Schizophr. Res. 2015 May 164: 100-8 |
|---|---|
| PMID | 25757715 |
| Title | Altered prefrontal cortical MARCKS and PPP1R9A mRNA expression in schizophrenia and bipolar disorder. |
| Abstract | We previously observed dendritic spine loss in the dorsolateral prefrontal cortex (DLPFC) fromschizophreniaand bipolar disorder subjects. In the current study, we sought to determine if the mRNA expression of genes known to regulate the actin cytoskeleton and spines correlated with spine loss. Five candidate genes were identified using previously obtained microarray data from the DLPFC fromschizophreniaand control subjects. The relative mRNA expression of the genes linked to dendritic spine growth and function, i.e. IGF1R, MARCKS,PPP1R9A, PTPRF, and ARHGEF2, was assessed using quantitative real-time PCR (qRT-PCR) in the DLPFC from a second cohort includingschizophrenia, bipolar disorder, and control subjects. Functional pathway analysis was conducted to determine which actin cytoskeleton-regulatory pathways the genes of interest interact with. MARCKS mRNA expression was increased in bothschizophreniaand bipolar disorder subjects.PPP1R9AmRNA expression was increased in bipolar disorder subjects. For IGF1R, mRNA expression did not differ significantly among groups; however, it did show a significant, negative correlation with dendrite length. MARCKS andPPP1R9AmRNA expression did not correlate with spine loss, but they interact with NMDA receptor signaling pathways that regulate the actin cytoskeleton and spines. MARCKS andPPP1R9Amight contribute to spine loss inschizophrenia和双相情感障碍their interactions, possibly indirect ones, with NMDA signaling pathways that regulate spine structure and function. |
| SCZ Keywords | schizophrenia |